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Image Search Results
Journal: Virology
Article Title: The low pH-dependent entry of avian reovirus is accompanied by two specific cleavages of the major outer capsid protein mu 2C.
doi: 10.1006/viro.1996.0235
Figure Lengend Snippet: FIG. 8. Entry-related cleavages of m2C are cell type- and virus strain-independent. (A) Radiolabeled, purified avian reovirus strain 176 (176) and mammalian reovirus serotype 3 Dearing (T3) were used in an internalization assay, as described in the legend of Fig. 3, using QT6 cells or L929 cells, respectively. Samples were harvested at t Å 0, 45, or 90 min after virus adsorption and analyzed by SDS–PAGE and autoradiography. The locations of individual viral proteins are indicated on the sides of the figure. (B) Radiolabeled, purified avian reovirus strain S1133 was used in an internalization assay, as described in the legend of Fig. 3, using QT6 cells (QT6) or Vero cells (VERO). Samples were harvested at t Å 15, 30, 60, or 90 min after virus adsorption and analyzed by SDS–PAGE and autoradiography. The locations of individual viral proteins are indicated on the sides of the figure.
Article Snippet: The mammalian reovirus serotype 3 (Dearing strain)Since enveloped viruses utilize the same mechanism was obtained from Patrick Lee (University of Calgary)to mediate the membrane interactions involved in both and was plaque purified and grown in murine L929 cells.virus entry and syncytium formation (Bentz, 1993),
Techniques: Virus, Purification, Adsorption, SDS Page, Autoradiography